Year: 2019 | Month: March | Volume 12 | Issue 1
In Vitro Propagation and Development of Salt Tolerant Lines of Edible and Medicinal Varieties of Coleus sp.
DOI:10.30954/0974-1712.03.2019.2
Abstract:
Coleus sp. is one of the most important tuber crops of South East Asia. Pollen grains being sterile in the plant, vegetative propagation are the only method to carry out reproduction. However morphological and genetic variability are relatively absent by normal vegetative reproduction. Thereby tissue culture techniques of micropropagation through axillary shoot proliferation, organogenesis and shoot embryogenesis can improve the genetic constitution of the crops. For axillary shoot proliferation, nodal explants and for organogenesis and callusing leaf explants of Coleus parviflorus and Coleus forskohlii were used. Furthermore salt tolerant lines were tried to raise using different concentration of NaCl with normal MS media. Morphological and biochemical changes associated with the in vitro regenerated types were investigated to maintain the “true to type” propagation. RAPD analysis to find out variation between DNA of both in vivo and in vitro plants were carried out using OPA 11 and OPB 07 primers. The rate of multiplication was found to be higher in media supplemented with NAA, BA and GA3. Callusing from leaf explants was found to be very low in media supplemented with only GA3. Bud break and shoot multiplication was affected by higher concentration of NaCl at 10gm/l and 20gm/l. Evaluation of biochemical constituents revealed no variation in dry matter, starch and sugar content of regenerants and conventionally grown plants. RAPD analysis exhibited no variation in DNA between the in vivo and in vitro plants
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